HB-EGF ELISA

HB-EGF (Heparin-binding EGF-like Growth Factor) Overview

Family and Function:

• Family: EGF (Epidermal Growth Factor) family.
• Receptor Interaction: Acts as a ligand for ErbB receptor tyrosine kinases, including EGF receptor (EGFR), ErbB2, and ErbB4.
• Roles: Involved in cell proliferation, differentiation, migration, and inflammatory responses.

Forms of HB-EGF:

1. ProHB-EGF (Transmembrane Form):
• Location: Embedded in the cell membrane.
• Function: The precursor form, which is proteolytically processed to release other forms of HB-EGF.
2. sHB-EGF (Soluble Form):
• Formation: Produced by proteolytic cleavage of ProHB-EGF at the cell surface.
• Function: This soluble form is involved in autocrine and paracrine signaling, influencing cell growth and inflammation.
3. HB-EGF-CTF (C-terminal Fragment):
• Location: Remains attached to the cell membrane after cleavage of ProHB-EGF.
• Function: The C-terminal fragment continues to interact with cell surface receptors and contributes to signaling processes.

Expression and Roles:

• Cell Types: Expressed in a variety of cell types including epidermal cells, cardiac myocytes, vascular endothelial cells, smooth muscle cells, and macrophages.
• Physiological Roles:
• Cell Proliferation and Differentiation: Promotes growth and differentiation of various cell types.
• Inflammatory Responses: Involved in modulating inflammation.
• Cancer Invasion and Metastasis: HB-EGF has been implicated in cancer progression and metastasis due to its role in promoting cell migration and proliferation.

Receptor for Diphtheria Toxin:

• Function: HB-EGF acts as a receptor for diphtheria toxin, which can utilize HB-EGF to enter cells.

Clinical and Research Implications:

• Drug Development: Due to its roles in cancer and cell proliferation, HB-EGF is a target for therapeutic development in oncology and other diseases involving aberrant cell growth.
• Research Applications: The HB-EGF ELISA kit specifically measures the form containing the propeptide, useful for research into HB-EGF’s role in various physiological and pathological processes.

ELISA Kit Details:

• Purpose: Measures the human HB-EGF form containing the propeptide.
• Intended Use: For research purposes only; not for clinical diagnostic procedures.
• Additional Notes: The ELISA kit provides a means to quantify HB-EGF levels and investigate its biological roles and potential as a therapeutic target.

This ELISA Kit from Tecan IBL International is for research use only.

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