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Cell Line Kit SF, 4D-Nucleofector X Kit, 12 reactions, 100 µL Nucleocuvette Vessels

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    Cell Line Kit SF, 4D-Nucleofector X Kit, 12 reactions, 100 µL Nucleocuvette Vessels
    Code: V4XC-2012

    For efficient transfection of difficult-to-transfect cell lines, e.g. K-562, Neuro-2A or RAW264.7, in the 4D-NucleofectorTM X Unit (100 µL format)

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    Product Description

    High transfection efficiencies in difficult-to-transfect cell lines. The SF Cell Line 4D-Nucleofector X Kit L is one of our three 4D-Nucleofector Kits that is suited for transfecting difficult-to-transfect cell lines in the 100 µL Nucleocuvette Vessel format when working with the 4D-Nucleofector X Unit.

    Applications

    • Transfection of cell numbers in the range of 2 × 105to 2 × 107 cells
    • Low throughput Applications

    Includes:

    • 2 x 0.675 mL SF Cell Line NucleofectorTM Solution
    • 2 x 0.15 mL Supplement 1
    • 50 µg pmaxGFPTM Vector (1 μg/μl in 10 mM Tris pH 8.0)
    • 12 x 100 µL NucleocuvetteTM Vessel
    • 12 x Single use pipettes

    This kit has been determined to be optimal for transfecting following cell types:

    • 293-F (FreeStyle), 32D
    • A-20, A2058, A375, A431, A549, AGS, ARPE-19
    • BetaTC/6, BxPC-3
    • C6, CCRF-CEM, CHO-K1, CHO-S (Invitrogen), Cos-1
    • EL4
    • FDC-P1
    • H9c2 (2-1), HEK293, Hel9217, HepG2, High 5, HL-60, HMC-1-8, HT-1080, HT-29, Hut-78, HUV-EC-C
    • IMR-32
    • 1
    • K-562
    • KG-1
    • L-428, LNCaP
    • MDA-MB-453, MDBK, Molt-4, MV-4-11
    • Nalm-6, NB-4, NCI-H1299 [H1299], Neuro-2a [N2a], NG108-15, NS0
    • P19, P815, PC-12, PC-3
    • Raw 264.7, RBL-1, RBL-2H3, Reh, Rin m5F, RPMI 8226
    • S2, S49, SaOS, SF9, SH-SY5Y, SK-BR-3, SK-N-AS, SK-N-MC, SK-N-SH, SK-OV-3, Sp2/0-Ag14, SW480
    • T2, T84, TF-1
    • U266B1, U-937
    • Vero

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