Most sensitive TMB peroxidase substrate for ELISA. Superior to ABTS and OPD. Develops blue color at 650nm, yellow when stopped at 450nm.
TMB 2-Component Microwell Peroxidase Substrate
Product Code: 5120-0053
Product Overview
SeraCare KPL TMB 2-Component Microwell Peroxidase Substrate Kit is the most sensitive chromogenic substrate available for peroxidase detection in ELISA applications. TMB (3,3',5,5'-tetramethylbenzidine) provides superior sensitivity compared to ABTS and OPD substrates, making it ideal for detecting low-abundance targets.
Key Features
- Most sensitive chromogenic peroxidase substrate
- More sensitive than ABTS and OPD
- Develops deep blue color measurable at 650 nm
- Turns yellow when stopped with acid (read at 450 nm)
- Two-component format for extended shelf life
- Suitable for ELISA and membrane blotting (with enhancer)
- ISO 13485-certified quality
Applications
- High-sensitivity ELISA assays
- Low-abundance target detection
- Antibody and antigen quantification
- Western blotting (with membrane enhancer)
- Research and IVD development
Technical Specifications
- Detection wavelength: 650 nm (unstopped), 450 nm (stopped)
- Substrate type: Chromogenic (soluble)
- Format: Two-component system - mix equal volumes before use
- pH range: 4.65-5.05 (mixed substrate)
- Storage: 2-8°C
- Shelf life: Minimum 1 year when stored properly
Quality Assurance
All SeraCare KPL products are manufactured under ISO 13485-certified quality systems, ensuring exceptional performance and consistent lot-to-lot reproducibility. Each product is thoroughly tested to meet strict quality specifications.
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