Code: LT07-610
Lonza PPiLight™ Inorganic Pyrophosphate Assay is a bioluminescence-based assay for sensitive quantification of inorganic pyrophosphate (PPi) in reactions.
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Lonza PPiLight™ Inorganic Pyrophosphate Assay is a bioluminescence-based assay for the sensitive quantification of inorganic pyrophosphate (PPi) in biochemical reactions. PPi is released as a by-product of many enzymatic reactions involving nucleoside triphosphates — including DNA and RNA polymerisation, aminoacyl-tRNA synthetases, and nucleotide addition by reverse transcriptases — making PPi measurement a powerful tool for real-time monitoring of enzymatic activity and sequencing-by-synthesis workflows.
Key Features
- Sensitive bioluminescence-based detection of inorganic pyrophosphate (PPi)
- Compatible with standard luminometers
- Enables real-time monitoring of nucleotide incorporation and enzymatic reactions
- 500-test kit for high-throughput applications
Applications
- Monitoring of DNA and RNA polymerase activity
- Pyrosequencing and sequencing-by-synthesis workflows
- Nucleotide addition and polymerisation kinetics assays
- Enzymatic reaction monitoring