LKTM010B Human Immunotherapy Luminex 25-plex from Bio-Techne. High performance validated multiplex immunoassay for research.
The LKTM010B Human Immunotherapy Luminex Performance Assay is a magnetic bead-based multiplex immunoassay manufactured by Bio-Techne (R&D Systems). This validated 25-plex fixed panel simultaneously quantifies immune checkpoint molecules, cytotoxic markers, and inflammatory cytokines for cancer immunotherapy in a single sample.
Analytes Detected
This 25-plex panel measures the following 25 analytes:
- CCL2/JE/MCP-1
- CCL3/MIP-1 alpha
- CCL4/MIP-1 beta
- CD40 Ligand/TNFSF5
- CXCL10/IP-10/CRG-2
- GM-CSF
- Granzyme B
- IFN-alpha 2/IFNA2
- IFN-gamma
- IL-1 alpha/IL-1F1
- IL-1 beta/IL-1F2
- IL-1ra/IL-1F3
- IL-2
- IL-4
- IL-6
- IL-8/CXCL8
- IL-9
- IL-10
- IL-12 p70
- IL-13
- IL-15
- IL-17/IL-17A
- IL-33
- PD-L1/B7-H1
- TNF-alpha
Key Features & Benefits
- Multiplex Efficiency: Measure 25-plex in one sample with minimal sample volume (<50 µL)
- Validated Performance: Correlated to gold standard Quantikine ELISA assays
- Optimized Diluents: Custom formulations for maximum sensitivity and recovery (70-130%)
- Quality Assured: Rigorous validation for specificity, precision, linearity, and recovery
- Fast Results: Complete assay in 3-5 hours
- Magnetic Beads: Simplified wash steps with magnetic plate separator
- Mass-Calibrated Standards: Consistent results across lots with Certificate of Analysis
Validated Sample Types
- Cell culture supernatants
- Serum
- Plasma (EDTA, heparin, citrate)
- Additional matrices may be validated - consult product datasheet
Research Applications
- Immuno-oncology research
- Car-t cell therapy monitoring
- Checkpoint inhibitor studies
- Nk cell function assessment
- T cell cytotoxicity
- Therapeutic efficacy monitoring
Technical Specifications
- Platform Compatibility: Luminex MAGPIX, FLEXMAP 3D, xMAP INTELLIFLEX, Luminex 200
- Detection Method: Sandwich immunoassay with Streptavidin-PE fluorescent detection
- Standards: Mass-calibrated recombinant protein standards with Certificate of Analysis
- Precision: Intra-assay CV <10%, Inter-assay CV <15%
- Linearity: Validated across assay range for all sample types
- Recovery: 70-130% for all validated sample matrices
- Kit Format: 96-well plate format, sufficient reagents for one plate
Kit Contents
- Premixed antibody-coated magnetic microparticles
- Premixed biotinylated detection antibody cocktail
- Streptavidin-Phycoerythrin conjugate
- Recombinant protein standards (lyophilized)
- Assay buffer
- Wash buffer concentrate
- Standard value card with COA values
Quality Control & Validation
Bio-Techne Luminex High Performance Assays undergo the most extensive validation in the industry. Each assay is tested for sensitivity (three-quarters of low standard), intra-assay precision, inter-assay precision, linearity of dilution, and spike recovery in all validated sample types. Every lot is QC tested to ensure consistent, reproducible results that correlate with Quantikine ELISA standards.