Lonza SeaKem® LE Agarose was the first and is still the world's most trusted agarose for nucleic acid electrophoresis.
Lonza SeaKem® LE Agarose was the first and is still the world's most trusted agarose for nucleic acid electrophoresis. Ideal for routine analysis of nucleic acids by gel electrophoresis and blotting, SeaKem® LE Agarose sharply resolves DNA and provides consistent resolution from lot to lot. This molecular biology grade agarose has no detectable DNase or RNase activity and forms strong gels with low background upon ethidium bromide, SYBR® Green, or GelStar® staining. Due to its low electroendosmosis (EEO), DNA has high electrophoretic mobility. SeaKem® LE Agarose is also recommended for protein gel electrophoresis applications such as Ouchterlony and radial immunodiffusion (RID).
Analytical Specifications
- Gelling temperature (1.5%): 36°C ± 1.5°C
- Melting temperature (1.5%): >90°C
- Gel strength (1%): >1,200 g/cm²
Key Features
- Wide resolution range: 100 bp – 23 kb
- Quality tested and certified for consistent lot-to-lot performance
- High gel strength
- Low EEO for high electrophoretic mobility
- Standard melting temperature
- No detectable DNase or RNase activity
Applications
- Standard DNA and RNA electrophoresis
- Southern and Northern blotting
- Protein gel electrophoresis (Ouchterlony, RID)