Lonza MetaPhor® Agarose is a high-resolution agarose that rivals polyacrylamide in resolving power for small DNA fragments.
Lonza MetaPhor® Agarose is a high-resolution agarose that rivals polyacrylamide in resolving power for small DNA fragments. MetaPhor® Agarose has twice the resolution capabilities of the finest-sieving agarose products — for example, a 200 bp DNA fragment can be separated from a 204 bp fragment. Using fast-running protocols, DNA differing in size by as little as 1% can be resolved in approximately 1.5 hours in a 20 cm horizontal or vertical gel format. MetaPhor® Agarose gels (2–4%) approximate the resolution of polyacrylamide gels (4–8%), making them ideal for resolving AMPFLPs, STRs, and tri- and tetranucleotide repeats without the handling hazards of acrylamide.
Analytical Specifications
- Gelling temperature (3%): ≤35°C
- Melting temperature (3%): ≤75°C (intermediate melting temperature)
- Gel strength (3%): ≥300 g/cm²
Key Features
- Polyacrylamide-equivalent resolution without acrylamide handling hazards
- Resolves DNA fragments differing by as little as 1% in size
- Quality tested and certified for consistent lot-to-lot performance
- High gel strength
- Low EEO
Applications
- Small PCR fragment analysis
- Microsatellite (STR) and AMFLP analysis
- Mutation detection
- Genotyping