Lonza IsoGel® Agarose is a standard melting temperature agarose specifically developed for isoelectric focusing (IEF) of proteins and separation of ampholytes.
Lonza IsoGel® Agarose is a standard melting temperature agarose specifically developed for isoelectric focusing (IEF) of proteins and separation of ampholytes. Unlike standard DNA-grade agarose, IsoGel® Agarose is formulated to be essentially free of charged groups that would otherwise interfere with the stable pH gradients required for successful IEF. Its very low electroendosmosis (EEO) eliminates convective disturbance of focused protein bands, providing sharp, reproducible results in IEF applications.
Key Features
- Optimised for isoelectric focusing (IEF) of proteins
- Very low EEO — prevents convective disturbance of focused bands
- Essentially free of charged groups that would disrupt pH gradients
- Quality tested and certified for consistent lot-to-lot performance
- Standard melting temperature for easy gel preparation
Applications
- Isoelectric focusing (IEF) of proteins
- Ampholyte separation
- Analytical protein electrophoresis