Efficient extraction of RNA >18 nt from FFPE samples. Tiangen’s kit ensures pure, DNA-free RNA for RT-PCR, qPCR, microarrays, and sequencing.
Tiangen miRNAprep Pure FFPE Kit - Highly efficient extraction of RNA > 18 nt from paraffin sections
Tiangen, one of the most cited brands for extraction kits, delivers advanced solutions for molecular biology. Formalin fixation can lead to cross-linking of RNA-RNA and RNA- protein in tissues, thus affecting the application of RNA in downstream experiments. The kit provides unique lysis and incubation conditions to reverse the crosslinking of RNA caused by formalin. After xylene deparaffinization, RNA can be effectively released from tissue sections by treatment with specially developed lysis buffer. The digestion of DNase I can ensure no genomic DNA contamination. RNA > 18 nt can be obtained after purification by silica matrix membrane. The product can be directly used in downstream experiments such as RT-PCR, RT-qPCR, Microarray analysis, etc.
Features
- High specificity: Specially designed for extracting miRNA from FFPE with optimized components.
- Simple and fast: High-quality miRNA can be obtained within 2 hours.
- Ultra-pure: Column purification to avoid gDNA and salt contamination.
- Publications: Trusted by scientists worldwide, Tiangen kits are backed by a high number of publications
Required Reagents
- Xylene, anhydrous ethanol
Applications
- RT-PCR
- RT-qPCR
- Northern Blot analysis
- Microarray analysis
- Sequencing of miRNA library