LKTM013B Human Growth Factor Luminex 21-plex from Bio-Techne. High performance validated multiplex immunoassay for research.
The LKTM013B Human Growth Factor Luminex Performance Assay is a magnetic bead-based multiplex immunoassay manufactured by Bio-Techne (R&D Systems). This validated 21-plex fixed panel simultaneously quantifies growth factors, chemokines, and regulatory molecules for cell proliferation and signaling in a single sample.
Analytes Detected
This 21-plex panel measures the following 21 analytes:
- CCL3/MIP-1 alpha
- CCL19/MIP-3 beta
- CD40 Ligand/TNFSF5
- CXCL2/GRO beta/MIP-2/CINC-3
- EGF
- FGF basic/FGF2/bFGF
- Flt-3 Ligand/FLT3L
- G-CSF
- Granzyme B
- IFN-beta
- IL-3
- IL-8/CXCL8
- IL-9
- IL-17E/IL-25
- IL-33
- PD-L1/B7-H1
- PDGF-AA
- PDGF-AB/BB
- TGF-alpha
- TRAIL/TNFSF10
- VEGF
Key Features & Benefits
- Multiplex Efficiency: Measure 21-plex in one sample with minimal sample volume (<50 µL)
- Validated Performance: Correlated to gold standard Quantikine ELISA assays
- Optimized Diluents: Custom formulations for maximum sensitivity and recovery (70-130%)
- Quality Assured: Rigorous validation for specificity, precision, linearity, and recovery
- Fast Results: Complete assay in 3-5 hours
- Magnetic Beads: Simplified wash steps with magnetic plate separator
- Mass-Calibrated Standards: Consistent results across lots with Certificate of Analysis
Validated Sample Types
- Cell culture supernatants
- Serum
- Plasma (EDTA, heparin, citrate)
- Additional matrices may be validated - consult product datasheet
Research Applications
- Angiogenesis research
- Wound healing and regeneration
- Stem cell research
- Cancer biology
- Cell signaling studies
- Tissue development
Technical Specifications
- Platform Compatibility: Luminex MAGPIX, FLEXMAP 3D, xMAP INTELLIFLEX, Luminex 200
- Detection Method: Sandwich immunoassay with Streptavidin-PE fluorescent detection
- Standards: Mass-calibrated recombinant protein standards with Certificate of Analysis
- Precision: Intra-assay CV <10%, Inter-assay CV <15%
- Linearity: Validated across assay range for all sample types
- Recovery: 70-130% for all validated sample matrices
- Kit Format: 96-well plate format, sufficient reagents for one plate
Kit Contents
- Premixed antibody-coated magnetic microparticles
- Premixed biotinylated detection antibody cocktail
- Streptavidin-Phycoerythrin conjugate
- Recombinant protein standards (lyophilized)
- Assay buffer
- Wash buffer concentrate
- Standard value card with COA values
Quality Control & Validation
Bio-Techne Luminex High Performance Assays undergo the most extensive validation in the industry. Each assay is tested for sensitivity (three-quarters of low standard), intra-assay precision, inter-assay precision, linearity of dilution, and spike recovery in all validated sample types. Every lot is QC tested to ensure consistent, reproducible results that correlate with Quantikine ELISA standards.