AmplideX Nanopore Carrier Plus Kit B uses long-read Nanopore sequencing to screen FMR1 CGG repeats for Fragile X syndrome carrier research.
AmplideX Nanopore Carrier Plus Kit B combines long-range PCR enrichment with Oxford Nanopore long-read sequencing to detect carrier variants across a multi-gene reproductive health panel. The kit runs on Oxford Nanopore MinION or GridION instruments with R10.4.1 flow cells and is analyzed using AmplideX Reporter software, requiring 40–100 ng of genomic DNA (gDNA) from blood, cheek swab, or saliva.
Kit B is one of four AmplideX Nanopore Carrier Plus Kits that together form a modular carrier screening panel. Order any combination of Kits A–D to build the coverage your workflow requires.
- Diseases Tested: Cystic Fibrosis, Spinal Muscular Atrophy, Fragile X Syndrome, α-Thalassemia, β-Thalassemia and Sickle Cell Disease, Congenital Adrenal Hyperplasia, Ehlers-Danlos Syndrome (Tenascin-X Deficiency), Gaucher Disease, Hemophilia A
- Technology: Long-range PCR enrichment with Nanopore long-read sequencing
- Format: 96 reactions per kit (RUO)
- Sample type: Genomic DNA from blood, cheek swab, or saliva
- Genes/targets: FMR1
- This kit covers: Fragile X syndrome carrier screening
- Full panel (Kits A–D combined): Cystic fibrosis, spinal muscular atrophy, Fragile X syndrome, alpha- and beta-thalassemia, sickle cell disease, congenital adrenal hyperplasia, Ehlers-Danlos syndrome (Tenascin-X deficiency), Gaucher disease, and hemophilia A
AmplideX Nanopore Carrier Plus Panel – Kit Coverage
| Kit | Genes | Conditions Covered |
|---|---|---|
| A | CFTR, SMN1, SMN2 | Cystic fibrosis, spinal muscular atrophy |
| B | FMR1 | Fragile X syndrome |
| C | HBA1, HBA2, HBB | Alpha-thalassemia, sickle cell disease |
| D | F8, GBA, CYP21A2, TNXB | Hemophilia A, Gaucher disease, congenital adrenal hyperplasia |