QuantideX qPCR JAK2 Kit detects the JAK2 V617F mutation by qPCR for myeloproliferative neoplasm (MPN) research from blood or marrow.
QuantideX qPCR JAK2 Kit is a multiplex real-time PCR (qPCR) assay that detects the JAK2 V617F mutation alongside an endogenous reference control in a single well, from whole blood or bone marrow aspirate. The assay achieves a limit of detection/quantification of approximately 0.0126–0.123% variant allele frequency (VAF) depending on DNA input (20–615 ng), and shares harmonized qPCR conditions with other QuantideX HemOnc kits for co-running on a single plate.
- Diseases Tested: Myeloproliferative Neoplasms (MPNs)
- Technology: Multiplex real-time qPCR
- Format: 60 reactions per kit (RUO), up to 49 samples per plate
- Sample type: Whole blood or bone marrow aspirate
- Applications: Myeloproliferative neoplasm (MPN) research, including polycythemia vera, essential thrombocythemia, and primary myelofibrosis