TIANGEN TIANamp Blood DNA Kit DP348 (50 preps): silica spin column gDNA from 0.1–1 ml whole blood. No phenol/chloroform. PCR-ready gDNA in under 30 min.
The TIANGEN TIANamp Blood DNA Kit (DP348) is a silica membrane-based spin column kit for rapid, reliable isolation of high-quality genomic DNA from 0.1–1 ml of anticoagulant whole blood. The kit uses a unique buffer system and TIANGEN's proprietary silica membrane spin column to efficiently lyse blood cells, remove haemoglobin, proteins, and PCR inhibitors, and elute pure, ready-to-use gDNA without phenol/chloroform extraction or alcohol precipitation.
The purified gDNA is of high molecular weight, high purity (OD₂₆₀/OD₂₈₀ 1.7–1.9), and immediately suitable for demanding downstream applications including PCR, restriction enzyme digestion, Southern blotting, and next-generation sequencing (NGS).
Key features:
- Silica membrane spin column technology — no phenol/chloroform, no ethanol precipitation
- Processes 0.1–1 ml anticoagulant whole blood per preparation
- Efficient removal of haemoglobin, proteins, and PCR inhibitors
- High-quality gDNA: OD₂₆₀/OD₂₈₀ 1.7–1.9; suitable for PCR, restriction digestion, Southern blotting, and NGS
- Typical processing time: under 30 minutes
- Kit stored dry at room temperature (15–30°C) for up to 12 months
- 50 preps per kit (DP348-02)
Applications:
- Genomic DNA extraction from human and animal whole blood
- PCR and qPCR template preparation
- Restriction enzyme digestion and Southern blotting
- NGS library preparation from blood-derived gDNA
- Genetic analysis, genotyping, and population genetics studies